Confocal microscopy is one of the most powerful tools used for the visualization of many cellular phenomena since it permits a great precision of observations. Obtained images show internal structures of the cells, two- and three-dimensional molecular interactions and in real time. To take advantage of the many possibilities of these complex instruments, GCRC members benefit from the expertise of our highly trained technicians. Their superb knowledge and attention to detail increases the efficiency of experimental procedures, all with the aim of establishing the best experimental conditions and minimalization of artifacts.
The GCRC Imaging Core Facility has currently the following microscopes available:
Pascal 1: Zeiss LSM Pascal on Axiovert 200 (routine one or two color confocal work)
Pascal 2: Zeiss LSM 5 Pascal on Axiovert 200 (routine one or two color confocal work)
Axiovert 1: Zeiss Axiovert 200 fully automated inverted microscope with multichannel epifluorescence, phase contrast, dark field and bright field imaging, and a Z-stack or timelapse as well as image deconvolution module.
Goodman Cancer Centre facility is an extension of the McGill Life Sciences Complex (LCS) Imaging Facility which mandate is to provide a state-of-the-art light microscopy imaging facility with advanced technical expertise in order to enhance the level of research for the community at McGill University and for the scientific community outside of McGill. The LCS Facility resides in the new Bellini Building and includes high-performance microscopy for live analysis and rapid automated imaging. The facility has local managers for each piece of equipment who are available for training, advice, and general assistance when using the microscopes. The facility director is also available, in particular for advice on fluorescent dyes, how to perform highly technical experiments like FRAP and FCS, live cell imaging, and image analysis.
A local manager is available for training, advice and general assistance in matters related to the use and operation of these microscopes.